| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 981 | Leptospira biflexa | TCACACCGGAAACTGCAGA | TTGCAGTGGCTGAACCGT | 59.17 | 59.81 | 186 | 90 |
100.00%
|
100.00%
|
| Primer 982 | Leptospira biflexa | AACTCACACCGGAAACTGCA | TTGCAGTGGCTGAACCGT | 60.11 | 59.81 | 189 | 90 |
100.00%
|
100.00%
|
| Primer 983 | Leptospira biflexa | TCACACCGGAAACTGCAGAA | TTGCAGTGGCTGAACCGT | 59.82 | 59.81 | 186 | 90 |
100.00%
|
100.00%
|
| Primer 984 | Leptospira biflexa | CTCACACCGGAAACTGCAG | TTGCAGTGGCTGAACCGT | 58.76 | 59.81 | 187 | 90 |
100.00%
|
100.00%
|
| Primer 985 | Leptospira biflexa | CTCACACCGGAAACTGCAGA | TTGCAGTGGCTGAACCGT | 60.25 | 59.81 | 187 | 90 |
100.00%
|
100.00%
|
| Primer 986 | Leptospira biflexa | CAACTCACACCGGAAACTGC | TTGCAGTGGCTGAACCGT | 59.69 | 59.81 | 190 | 90 |
100.00%
|
100.00%
|
| Primer 987 | Leptospira biflexa | ACTCACACCGGAAACTGCAG | TTGCAGTGGCTGAACCGT | 60.53 | 59.81 | 188 | 90 |
100.00%
|
100.00%
|
| Primer 988 | Leptospira biflexa | CGAAGTGTGGCTTGGTATGC | TGCCAGGCCATTCCTTCAG | 59.55 | 60.00 | 185 | 90 |
100.00%
|
100.00%
|
| Primer 989 | Leptospira biflexa | TGGAATGAGATCGTCGGAGAG | TGGGTATGCGATGGCGTT | 59.05 | 59.41 | 159 | 89 |
100.00%
|
100.00%
|
| Primer 990 | Leptospira biflexa | TGGTGCCGTGATCACTGTT | ACGGTAAGCGGAGGAAGGT | 59.55 | 60.61 | 298 | 89 |
100.00%
|
100.00%
|